Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Gey's Balanced Salt Solution liquid, sterile-filtered, suitable for cell culture | 500ML
Gey's Balanced Salt Solution liquid, sterile-filtered, suitable for cell culture | 500ML
Research Products International Corp TRIS ACETATE ULTRA PURE, 500 GRAMS
Moisture: <0.3%
pH (0.1M, H2O) @25°C: 6.0-7.0%
Heavy Metals: 0.001%
Research Products International Corp TRIS BUFFERED SALINE, 10X SOLUTION, 4 LITER
TBS 10X Solution (Tris Buffered Saline) is a concentrated buffered salt solution containing Tris and sodium chloride. It maintains pH stability and provides ionic strength for biological experiments. Tris acts as a buffering agent to keep pH consistent, while sodium chloride mimics physiological conditions. Dilute 10X solution with distilled water to prepare working concentrations (e.g., 1X). TBS is widely used in protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques, ensuring stable and reproducible results across diverse applications.
General Laboratory Products 2mL 0.85 per. Saline, 100/cs
2mL 0.85 per. Saline with Shelf Life of 1 yr.
Cell Signaling Technology Tris-Glycine SDS Running Buffer (10X) 1000 ml
Tris-Glycine SDS Running Buffer (10X) 1000 ml
![Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 4.5ml Pre-Filled Tubes, Sterile, 36 Tubes per Case,10ml Tube Size](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10900-36.0.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 4.5ml Pre-Filled Tubes, Sterile, 36 Tubes per Case,10ml Tube Size
Ready to use sterile 1X solution, supplied in sterile pre-filled screw cap tubes. Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. Each tube contains 4.5 ml PBS 1X Sterile Solution, packaged in a sealed white cardboard freezer box with a 6 x 6 divider. PBS is supplied in 10 ml screw cap tubes that are 3.5 “ high (cap on). Each case holds 36 pre-filled tubes.
Convenient 4.5ml of PBS per tube
Sterile
36 x 4.5 ml
Kd Medical Inc TRIS GLYC SDS BUFFER, 10X, 4L
10X Tris Glycine SDS is used for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets. One 4 liter cubitainer.
Kd Medical Inc TRIS GLYCIN TRANS BUF 10X, 1L
Tris-Glycine Transfer Buffer (10X) is used as a transfer buffer during western blotting applications. 10X Tris Glycine is concentrated and ready to be diluted to a 1X working solution. One 1 liter bottle.
Kd Medical Inc PBS 10X, PH 7.4, 500ML
10X PBS is concentrated is and ready to be diluted to a 1X working solution. Used as a wash buffer in several research applications. One 500ml bottle.
Kd Medical Inc TRIS BUFFER, 2M, PH 7.5, 500ML
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. Tris Solution, 2M, provides a convenient stock solution for preparing Tris buffers> One 500 ml bottle.
Kd Medical Inc TE 1X, PH 8.0, 500 ML
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. One 500ml bottle.
![eMolecules Ambeed / (4-(6-Methyl-1245-tetrazin-3-yl)phenyl)methanamine hydrochloride / 1g / 666597771 / A1152138 / / 1596117-29-1 / [null] / 237.690 / C10H12ClN5](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502475470.JPG-250.jpg)
eMolecules Ambeed / (4-(6-Methyl-1245-tetrazin-3-yl)phenyl)methanamine hydrochloride / 1g / 666597771 / A1152138 / / 1596117-29-1 / [null] / 237.690 / C10H12ClN5
Ambeed / (4-(6-Methyl-1245-tetrazin-3-yl)phenyl)methanamine hydrochloride / 1g / 666597771 / A1152138 / / 1596117-29-1 / [null] / 237.690 / C10H12ClN5
Growcells PBS 20X 1L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g