Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659953.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 500 G
PIPES (Piperazine N,N' bis(2 ethanesulfonic acid)) is a biochemical reagent commonly used to prepare buffer solutions that maintain a stable pH environment in various laboratory experiments. This 500g formulation is perfect for high-volume applications in research involving protein purification, enzyme assays, and microbiological cultures. PIPES provides reliable pH stability around 7.0 and is widely used in studies involving proteins, cells, and biological macromolecules. Its high buffering capacity makes it ideal for maintaining consistent experimental conditions during cell growth, enzymatic reactions, and protein analysis, ensuring accurate and reproducible results.
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660241.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G
HEPES, Sodium Salt is a biochemical reagent supplied in a 250g package, offering a reliable buffer for experiments requiring precise pH control. This sodium salt form of HEPES maintains a stable pH of 6.8–8.2, which is essential for a variety of biochemical and biological applications such as cell culture, protein analysis, and enzymatic assays. HEPES sodium salt is known for its excellent buffering capacity, ensuring that pH remains constant even during extended incubation periods. It is widely used in research and laboratory environments due to its low toxicity, making it a preferred choice for maintaining stable conditions during biological experiments.
Medchemexpress LLC MOPS | 1132-61-2 | 99.95% | 10 G
MOPS is a buffering agent used in biological applications. It functions to maintain the pH of mammalian cell culture media. Additionally, it has been shown to significantly enhance the thermal stability of BSA.
- Functions as a buffering agent in biology.
- Maintains the pH of mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661238.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 5 Kilograms
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt), 5 Kilograms, is a highly specialized biochemical reagent used primarily for preparing buffers in laboratory research. PIPES is particularly valuable for maintaining a stable pH in biological systems, including enzyme reactions and cell culture environments. It is commonly used in electrophoresis, chromatography, and as a component in molecular biology protocols. The large 5 kg volume makes it ideal for high-throughput laboratories and large-scale biochemical experiments. PIPES is known for its exceptional buffering capacity in the physiological pH range, making it an essential tool in many laboratory applications.
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 25 G
ADA disodium is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, especially in the preparation of enzyme buffers.
- Derived from adenosine deaminase
- Used in biochemical research
- Ideal for enzyme buffer preparation
LICORbio Odyssey MES SDS Running Buffer Powder (pack of 5)
Odyssey Mini Gels are polyacrylamide gels designed to separate a wide range of protein sizes via PAGE or SDS-PAGE. Each box contains 10 pre-cast gels. Our gels feature larger well volumes for easier sample loading and are cast in a neutral pH buffer to increase gel stability. They also run at neutral pH, which minimizes protein modification compared to Tris-glycine gels. Odyssey Mini Gels are available in several gradients with 10, 12, or 15 numbered wells. Our gels feature a shelf life of up to 6 months at room temperature and 12 months at 2–8C.
Dojindo Molecular Technologies Inc TAPS (25G), Good's buffer, CAS: 29915-38-6, pka=8.4, Active pH 7.7-9.1, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
eMolecules 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 20656-08-0 | MFCD00141691 | 1g
Matrix Scientific | 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 1g | 389721058 | 45569 | 95.000 | 20656-08-0 | MFCD00141691 | 234.080 | C9H9Cl2NO2
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Teknova 50mM MES 1M Sodium Chloride
50mM MES, 1M Sodium Chloride, pH 6.0, 1000mL, Endotoxin Tested, Sterile. 8 pack.
